By Kan Wang
A top-quality number of Agrobacterium-mediated transformation concepts for state of the art plant genetic engineering, practical genomic research, and crop development. quantity 1 info the main up to date innovations on hand for twenty-six plant species drawn from cereal vegetation, commercial crops, legume crops, and vegetable vegetation, and offers a variety of tools for introducing DNA into 3 significant version plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors additionally define the elemental tools in Agrobacterium manipulation and methods for vector building. quantity 2 comprises one other thirty-three confirmed strategies for root crops, turf grasses, woody species, tropic vegetation, nuts and end result, decorative vegetation, and medicinal crops. extra chapters supply equipment for introducing DNA into non-plant species, reminiscent of micro organism, fungi, algae, and mammalian cells. The protocols persist with the winning tools in Molecular Biology™ sequence layout, each one delivering step by step laboratory directions, an advent outlining the rules at the back of the process, lists of the mandatory gear and reagents, and tips about troubleshooting and fending off identified pitfalls.
Read or Download Agrobacterium Protocols, Second Edition: Volume I (Methods in Molecular Biology Vol 343) PDF
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Additional info for Agrobacterium Protocols, Second Edition: Volume I (Methods in Molecular Biology Vol 343)
Quite often, a reporter vector may be a good starting material for various gene constructs, as experimental vectors may be obtained simply by replacing one or more components in the reporter vector with appropriate DNA fragments. Useful tips related to vector construction are given in Notes 12–18. 1. Obtain plasmids and other DNA fragments necessary for constructions of vectors from appropriate sources. 2. Combine the bacteria-selectable marker and the plasmid replication functions for E. coli.
References 1. Lippincott, J. A. and Lippincott, B. B. (1975) The genus Agrobacterium and plant tumorigenesis. Annu. Rev. Microbiol. 29, 377–405. 2. Allen, O. N. and Holding, A. J. (1974) Genus II. Agrobacterium, in Bergey’s Manual of Determinitive Bacteriology, 8th ed. (Buchanan, R. E. and Gibbons, N. ), Williams & Wilkins, Baltimore, pp. 264–267. 3. Kerr, A. (1992) The genus Agrobacterium, in The Prokaryotes, Vol. , Truper, H. ), SpringerVerlag, New York, pp. 2220–2235. 4. Snell, J. J. S. (1984) General introduction to maintenance methods, in Maintenance of Microorganisms: A Manual of Laboratory Methods (Kirsop, B.
Thus, the magnesium source should be prepared and autoclaved separately from the phosphate source (6). Also, note that an acidic solution of sucrose is subject to hydrolysis when heated (6). This is of particular importance when preparing media for analysis of A. tumefaciens virulence genes, as glucose, but not sucrose will act as a stimulant of vir gene induction (8). 6. AB* medium is a derivative of AB medium (9). With the addition of a phenolic inducer, AB* becomes AB*I (AB* induction medium) for use as an inducing medium for A.