Download Advances in Biochemical Engineering, Volume 10 by Wayne H. Pitcher Jr. (auth.) PDF

By Wayne H. Pitcher Jr. (auth.)

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Once the disguised rate constants are known the rates of reaction can be calculated from the derived equations. If the intrinsic first order rate constants kl and k2 are known, and estimates of diffusion coefficient (D) and mass transfer coefficient (kb) are available, the system performance may be predicted for a given geometry. Concentration prof'fles with time can be derived and plots for different conditions constructed. For short reaction times a large resistance by the membrane gives selectivity in favour o f P2- A small resistance by the membrane (low Thiele modulus q~l ) favours P1 production.

84. 85. 86. 87. 88. 89. 90. 91. 92. 93. 94. 95. 96. 97. 98. 99. 100. 101. : Can. J. Chem. Eng. 50, 162 (1972) Pitcher, W. H. : Sc. D. Thesis, Mass. Inst. of Tech. (1972) Satterfield, C. , Colton, C. , Pitcher, W. H. : AIChE J. 19,628 (1973) Hamilton, B. , Gardner, C. , Colton, C. : AIChE J. 20, 503 (1974) Frouws, M. , DeWilt, H. G. : Bioteehnol. Bioeng. , Laidler, K. : Biotechnol. Bioeng. 16, 99 (1974) Bunting, P. , Laidler, K. : Biotechnol. Bioeng. 16, 119 (1974) Ngo,T. , Laidler, K. L: Bicchim.

4 Immobilized Whole Cells Considerable interest has arisen in recent years in the use of whole cells, either entrapped in matrices, bound to particles, or as aggregates, for the production of various materials. The use of whole cells often obviates the difficulty of enzyme isolation and sometimes overcomes the problems of enzyme instability. There can often be more specific advantages when a given process requires a number of enzyme reactions, as the cellular enzyme systems will perhaps contain the enzymes organised in the requisite pathways.

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